HSP47在单侧输尿管梗阻大鼠模型肾脏组织的表达及作用

Expression of HSP47 in renal tissues and its role in unilateral ureteral obstruction rats

目的:观察HSP47在单侧输尿管梗阻(unilateral ureteral obstruction,UUO)大鼠模型肾脏组织的表达情况,探讨HSP47在肾小管间质纤维化中的作用。方法:将SD大鼠随机分为模型组(UUO组)和假手术组(Sham组),每组8只。采用单侧(左)输尿管结扎方法,建立梗阻性肾小管间质纤维化大鼠模型。术后第14天处死动物。观察两组间蛋白尿和血肌酐水平,运用HE和Masson染色观察肾脏组织形态学改变,采用免疫组织化学方法检测HSP47、胶原Ⅳ(CollagenⅣ)的表达。结果:与Sham组比较,UUO组大鼠血肌酐水平升高,蛋白尿差异无统计学意义。光镜下UUO组大鼠肾组织出现不同程度的肾小管扩张、间质炎症细胞浸润,间质面积增宽、部分肾小管萎缩、肾小管上皮细胞空泡样变性。Masson染色结果显示UUO组大鼠肾组织胶原在肾小管周围、间质区显著增多。免疫组织化学结果显示UUO组大鼠肾组织HSP47表达显著增加,在肾小管周围、间质区表达增加最明显;CollagenⅣ表达显著增加,在肾小管基底膜、间质区表达增加最明显。结论:HSP47在UUO大鼠模型肾脏组织中表达上调,且与胶原表达部位一致,提示HSP47促进肾间质纤维化,且该作用与其促进胶原蛋白的合成有关。

Objective To evaluate the expression of HSP47 in renal tissues of unilateral ureteral obstruction（UUO） rat model and to determine its role in renal interstitial fibrosis.Methods Sprague-Dawley male rats were randomly divided into Sham and UUO groups（each group：n=8）.A UUO model was established by ligation of the left ureter.Rats were killed on the Day 14 after the surgery.In both groups,the proteinuria and serum creatinine levels were examined.Renal morphological changes were observed by Masson＇s trichrome stain.Expression of HP47 and CollagenⅣ were detected by immunohistochemistry.Results Compared with the Sham group,the serum creatinine levels increased in the UUO group,but there was no significant difference in proteinuria levels between 2 groups.In the UUO group,various degree of tubular dilatation or atrophy,infiltration of inflammatory cells in interstitial areas,expansion of interstitial space and bubble-like degeneration of tubular epithelial cells were observed.The results of Masson＇s trichrome stain showed that collagen expression was significantly increased around the renal tubule and in interstitial areas in the UUO rats.Immunohistochemistry assay revealed that the expression of HSP47 and Collagen Ⅳ significantly increased in the renal tissue of UUO rats,especially around the renal tubule and in interstitial areas.Conclusion The HSP47 expression increases in the kidney of UUO rats accompanied by the increased collagen expression,suggesting that HSP47 may be involved in renal interstitial fibrosis through promoting the biosynthesis of collagen.