摘要
目的了解临床常见病原菌的分布及耐药现状,为临床合理使用抗生素提供参考依据。方法采用纸片扩散法进行抗生素敏感性试验。结果共分离病原菌3 202株,其中革兰阴性杆菌占78.8%,革兰阳性球菌占15.7%,真菌占5.5%。大肠埃希菌、肺炎克雷伯菌中产超广谱β-内酰胺酶(ESBLs)检出率分别为68.4%和58.3%,葡萄球菌中耐甲氧西林金黄色葡萄球菌(MRSA)和耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)的检出率分别为53.6%和61.7%。碳青霉烯类抗生素对大肠埃希菌、肺炎克雷伯菌高度敏感,敏感率为100%,而对铜绿假单胞菌、鲍曼不动杆菌抗菌活性明显降低,敏感率为47.7%~71.6%,革兰阳性球菌对糖肽类和利奈唑胺抗生素高度敏感。结论细菌耐药性不断增强,应及时监测病原菌及耐药性变化趋势,加强抗生素合理使用。
Objective To investigate the pathogenic bacteria distribution and antibiotic resistance of clinical isolates from our hospital in 2009,and povide a basis for clinical treatment.Methods Antibiotic susceptibility tests were performed according tO K-B method.Results were analyzed according to CLSI 2009.and corresponding results were analyzed with WHONET 5.4 software.Resuits A total of 3202 clinical isolates were collected,of which Gram-negative organism,Gram-positive cocci and fungi accounted for 78.8%,15.7%and 5.5%,respectively.The ESBLs producing strains of Escherichia coli and Klebsiella pneumoniae accounted for 68.4%and 58.3%,respectively.Meticillin resistant strains of Staphylococcus aureus and coagulase negative Staphylococcus accounted for 53.6%and 61.7%,respectively.Escherichia coli and Klebsiella pneumoniae showed 100% susceptibility to carbopenems.But the susceptibility of Pseudomonas aeruginosa and Acinetobacter to carbopenems reduced to 47.7%~71.6%.Gram-positive cocci were highly sensitive to glycopeptides and linezolid.Conclusion Bacterial resistance increased,we should monitor pathogens and resistance change timely and strengthen the rational use of antimicrobial agents.
出处
《国际检验医学杂志》
CAS
2010年第6期568-570,572,共4页
International Journal of Laboratory Medicine
关键词
抗菌药
抗药性
细菌
革兰氏阳性球菌
革兰氏阴性菌
Anti Bacterial Agents
Drug Resistance,Bacterial
gram positive cocci
gram negative bacteria