抗痨胶囊剂体内外抗肺结核作用研究

Research of effects on anti-pulmonary tuberculosis of anti tuberculosis capsule in vitro and in vivo

目的体内外评价抗痨胶囊防治肺结核作用。方法运用试管法观察两种制剂体外对结核菌生长的影响,运用结核豚鼠观察药物体内对结核病的防治作用。结果抗痨胶囊体外对人型H37RV结核杆菌、牛型分枝杆菌及耐异烟肼、耐利福平、耐链霉素和联合耐异烟肼、利福平及链霉素结核杆菌的MIC和MBC分别为0.5mg/ml和5mg/ml、10mg/ml和大于20mg/ml、5mg/ml和10mg/ml、5mg/ml和10mg/ml、10mg/ml和20mg/ml。体内抗结核实验结果显示:抗痨胶囊可显著减少结核豚鼠肺脏的结核结节及肺脏和脾脏的结核菌数量,同时还可减轻结核豚鼠肝脏、脾脏及肺脏的炎性反应。并可显著减少豚鼠脾脏的结核结节。结论上述结果显示,抗痨胶囊对肺结核有一定的防治作用,有一定的开发前景。

Objective To estimate effects on anti-pulmonary tubenrculosis of anti tuberculosis capsule in vitro and in vivo.Methods Observe the growing effects of the two pharmaceutics on tubercle bacillus by test tube method in vitro,and the preventive and therapeutic effects of the drugs on guinea pigs’modles in vivo.Results MIC and MBC of anti tuberculosis capsule on H37RV tubercle bacillus,mycobacterium bovis,tubercle bacillus resistance to isoniazid,rifampicin,streptomycin,and tubercle bacillus combine resistance to isoniazid,rifampicin,streptomycin are 0.5 mg/ml and 5 mg/ml、10 mg/ml and〉 20 mg/ml、5 mg/mland 10 mg/ml、5 mg/ ml and 10 mg/ml、10 mg/ml and 20 mg/ml in vitro.In vivo test results showed that anti tuberculosis capsule could decrease significantly the tubercular nodule of lung and the amount of tubercle bacillus in the lung and splenic of guinea pigs,and alleviate inflammatory reaction in liver,spleen and lung of guinea pig,and decrease significantly the tubercular nodule of spleen.Conclusion The above results showed that anti tuberculosis capsule has some preventive and therapeutic effects on pulmonary tubereulosis,and has some exploitation perspective.