氯乙烯作业工人淋巴细胞DNA损伤的研究

Studies on DNA Damage in Human Lymphocytes of Workers Exposed to Vinyl Chloride

目的探讨用单细胞微量凝胶电泳测试方法（ＭＧＥ）检测氯乙烯对人体外周血淋巴细胞ＤＮＡ损伤。方法选择５３名氯乙烯作业工人和５５名非氯乙烯接触工人用ＭＧＥ方法测定外周血淋巴细胞ＤＮＡ损伤的状况。结果氯乙烯作业工人ＤＮＡ损伤细胞数明显高于对照组（Ｐ＜００１）。结论氯乙烯可引起人体淋巴细胞ＤＮＡ链的断裂，单个细胞微量凝胶电泳技术是一项敏感的测试人体细胞ＤＮＡ损伤的方法。

Objective To detect DNA damage in human peripheral lymphocytes of workers exposed to vinyl chloride with microgel single cell electrophoresis(MGE).Methods Fifty three workers exposed to vinyl chloride and 55 non exposed controls were selected.DNA damage in their lymphocytes were detected by MGE.Results Number of lymphocytes with DNA damage was significantly higher in the exposed workers than that in non exposed (P<0 001).Conclusion Vinyl chloride can cause cleavage of DNA strand in human lymphocytes,and MGE is a more sensitive method to detect DNA damage in human lymphocytes.