乙醛对大鼠胚胎肢芽细胞增殖分化的影响研究

Studies on Proliferation and Differentiation of Embryo Limb Bud Cell Induced by Acetaldehyde in Rats

目的研究乙醛的发育毒性和致畸性。方法从１３天龄大鼠胚胎中分离肢芽细胞，体外施予不同剂量的乙醛（０１，０２，０５，２０，８０，１６０，３２０ｍｍｏｌ／Ｌ）连续培养５天，根据肢芽细胞蛋白质合成和集落形成抑制水平反映乙醛的致畸性。结果乙醛在０１ｍｍｏｌ／Ｌ即能影响肢芽细胞的增殖分化，而在２０ｍｍｏｌ／Ｌ以上时细胞则大量死亡，显示其细胞毒性。其ＩＰ５０，ＩＤ５０分别为６８４ｍｍｏｌ／Ｌ，５５０ｍｍｏｌ／Ｌ。增殖分化抑制曲线经拟合后判断乙醛是一种非特异性增殖分化抑制物。结论乙醛具有发育毒性作用和潜在的致畸作用。

Objective Acetaldehyde is a widely used chemical,but its teratogenecity has been controversial.To study its effects on development and teratogenecity and their mechanisms,culture of rat embryo limb bud cells was used to examine their proliferation and differentiation.Methods Embryo limb bud cells were separated from rats of 13 days old and cultivated in 0 1,0 2,0 5,2 0,8 0,16 0 and 32 0 mmol of acetaldehyde,respectively,for five days in vitro.The inhibition of protein synthesis and chondrogenesis was observed.Results Acetaldehyde at a concentration of 0 1 mmol could affect the proliferation and differentiation of the cultured cells,and that at 2 0 mmol over could cause cell death.IP 50 of acetaldehyde was 6 84 mmol and ID 50 5 50 mmol.Conclusion Acetaldehyde is a chemical with developmental toxicity and potential teratogenecity.