摘要
目的研究乙醛的发育毒性和致畸性。方法从13天龄大鼠胚胎中分离肢芽细胞,体外施予不同剂量的乙醛(01,02,05,20,80,160,320mmol/L)连续培养5天,根据肢芽细胞蛋白质合成和集落形成抑制水平反映乙醛的致畸性。结果乙醛在01mmol/L即能影响肢芽细胞的增殖分化,而在20mmol/L以上时细胞则大量死亡,显示其细胞毒性。其IP50,ID50分别为684mmol/L,550mmol/L。增殖分化抑制曲线经拟合后判断乙醛是一种非特异性增殖分化抑制物。结论乙醛具有发育毒性作用和潜在的致畸作用。
Objective Acetaldehyde is a widely used chemical,but its teratogenecity has been controversial.To study its effects on development and teratogenecity and their mechanisms,culture of rat embryo limb bud cells was used to examine their proliferation and differentiation.Methods Embryo limb bud cells were separated from rats of 13 days old and cultivated in 0 1,0 2,0 5,2 0,8 0,16 0 and 32 0 mmol of acetaldehyde,respectively,for five days in vitro.The inhibition of protein synthesis and chondrogenesis was observed.Results Acetaldehyde at a concentration of 0 1 mmol could affect the proliferation and differentiation of the cultured cells,and that at 2 0 mmol over could cause cell death.IP 50 of acetaldehyde was 6 84 mmol and ID 50 5 50 mmol.Conclusion Acetaldehyde is a chemical with developmental toxicity and potential teratogenecity.
出处
《中国工业医学杂志》
CAS
1999年第1期16-18,共3页
Chinese Journal of Industrial Medicine