氧化型染发剂遗传毒性作用的实验研究

Study on Genotoxicity of Oxidative Hair dyes

采用ＳＯＳ／Ｕｍｕ试验对氧化型染发剂中的两种主要成分双氧水（Ｈ２Ｏ２）和对苯二胺（ＰＰＤ）及二者混合物的遗传毒性作进一步探讨。结果表明：ＰＰＤ无直接遗传毒性，但空气氧化可使ＰＰＤ致遗传毒作用的危险性增强；Ｈ２Ｏ２有较强的直接遗传毒性并有剂量反应关系（ｒ＝０．９８１，Ｐ＜０．０１）。用不同浓度的Ｈ２Ｏ２（０～０．１２％）氧化２．５％的ＰＰＤ，反应混合物的遗传毒性有所增高（ｒ＝０．９８７，Ｐ＜０．０１）；若用０．０３％的Ｈ２Ｏ２氧化不同浓度的ＰＰＤ（０～２．５％），排除混合物中残余Ｈ２Ｏ２的遗传毒性作用，反应混合物的遗传毒作用也明显增高（ｒ＝０．９９４，Ｐ＜０．０１）。由此证明氧化型染发剂中。

The genotoxicity of two main components of oxidative hair dyes,such as p phenylenediamine(PPD)and hydrogen peroxide(H 2O 2),and their mixture were determined by SOS/Umu assay to confirm the genotoxic origin of oxidative hair dyes.The results showed that purified PPD had no direct genotoxicity,but when it was oxidized in air,the risk of genotoxicity of PPD might be increased.Hydrogen peroxide had strong direct genotoxicity and showed a dose respons relationship ( r=0.981,P<0 01 ).When H 2O 2 with different concentration (0￣0.12%) was mixed with 2 5% PPD,genotoxicity of their mixture increased ( r=0.987,P<0 01 ).When PPD with different concentrations (0￣2 5%) was oxidized by 0 03% H 2O 2,genotoxicity of the mixture from which the genotoxicity of H 2O 2 residue was eliminated,also increased ( r=0 994,P<0 01 ).It is concluded that the genotoxicity of oxidized hair dyes was not only caused by H 2O 2,but also by the oxidized product of PPD by H 2O 2.