摘要
目的:优化柴芩清肝方的提取工艺。方法:建立柴芩清肝方中黄芩苷、甘草苷和芍药苷3种成分同时测定的HPLC方法。采用L9(34)正交设计法,以黄芩苷、甘草苷、芍药苷3种成分含量综合评分法进行数据分析,对柴芩清肝方的提取次数、提取时间和加水量3因素进行优化。结果:3因素中只有提取次数有显著影响,最佳提取工艺为A3B1C3。结论:多成分评价法优选柴芩清肝方提取工艺能较好地保证制剂的质量。
Objective:To optimize the extraction technology of Chaiqin Qinggan Formular.Method:A HPLC method was established to simultaneously determine the contents of baicalin,paeoniflorin and glycyrrhizin in the extractions of Chaiqin Qinggan Formular.Based on the orthogonal design of L9(3^4) and the weighted method,three factors including the multiple water,decocting time,and extracting times were evaluated.Result:Significant effect was observed in extracting times.The best extraction process is A 3 B 1 C 3 .Conclusion:The extraction technology optimized with multiple components evaluation is reasonably practicable.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第7期10-12,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
黑龙江省科技攻关项目资助(GC08C322)
关键词
柴芩清肝方
正交设计
黄芩苷
芍药苷
甘草苷
Chaiqin Qinggan formular
orthogonal design
baicalin
paeoniflorin
glycyrrhizin