摘要
目的:研究红花多糖的提取及含量测定,为开展红花多糖的抗肿瘤作用机制提供理想药物。方法:采用水提醇沉法从红花药材中提取并分离出红花粗多糖,用Sevage法除去蛋白质,过氧化氢脱色;采用苯酚-硫酸显色,用分光光度法测定多糖含量。结果:红花多糖的浓度与吸光度在5~50μg·mL-1呈良好的线性关系,回归方程:Y=0.01749X-0.03165,r=0.9994。多糖质量分数为96.048%。结论:含量测定方法操作简便,灵敏度高,稳定,重现性好。该方法可为开展红花多糖的抗肿瘤作用研究提供理想的实验药物。
Objective:To study the extraction and assaying of Safflower polysaccharide(SPS) ,and to provide suitable experimental drug specimen for exploreing the anti-carcinoma effect of SPS.Method:Water extracting and ethanol precipitating methods were applied to extract polysaccharide from Safflower.By Sevage method,protein can be removed,and by oxidation method to decolor the polysaccharide.After phenol-sulphuric acid coloring,the polysaccharide content was determined by the method of absorption spectrometry.Result:The standard curve was liner in the concentration range of 5~50μg·mL^-1 with correlation coefficient of r=0.9994,the linear regression equation was Y=0.01749X-0.03165,the contents of polysaccharide from Safflower was 96.048%.Conclusion:This methods have advantages of simple operation,good stability,high accuracy and good reproducibility.This extraction technology could be used to extract Safflower polysaccharide for studies on its anticarcinoma effect.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第7期19-21,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
红花多糖
分光光度法
提取
纯化
含量测定
safflower polysaccharide (SPS)
spectrophotography
extraction
purification
assaying