HPLC测定养阴清肺颗粒中哈巴俄苷的含量

Determination of Harpagoside in Granules of Yangyin Qinfei by RP-HPLC

目的:建立养阴清肺颗粒中哈巴俄苷的测定方法。方法:采用反相高效液相色谱法,HypersilODS(4.6mm×250mm,5μm)柱,流动相为乙腈和1%醋酸水溶液,线性梯度洗脱(15∶85～60∶40)(0～25min),流速1mL·min-1,检测波长278nm,柱温25℃。结果:哈巴俄苷浓度在0.61～12.21μg线性关系良好,r=0.9999,平均回收率为97.02%,RSD为0.6%(n=9)。结论:该方法分离效果好,简便准确,可用于养阴清肺颗粒中哈巴俄苷的含量测定,以控制其质量。

Objective：Objective To determine the content of harpagoside in granules of Yangyin Qinfei. Method：A Rp-HPLC method was established.The chromatographic column was Hypersil ODS（4.6 mm×250 mm） ,and acetonitile-water（containing 1%acetic acid）（15∶85～60∶40）（0～25 min） was wsed as mobile phase.The flow rate was 1 mL·min^-1,the detection wavelength was at 278 nm,and the column temperature was 25 ℃.Result：The average recovery of harpagoside was 97.02%,and RSD was 0.6%（n=9） .Conclusion：The method established can be used to determine the content of Harpagoside in Granules of Yangyin Qinfei.