百合多糖铁复合物的合成及其铁含量的测定

Synthesis of Lily Polysaccharide-Fe (Ⅲ) Complex and Determination of Fe (Ⅲ)

目的:从百合中提取百合多糖,后与铁(Ⅲ)合成百合多糖铁复合物(LPC),建立LPC中铁含量的测定方法。方法:热水浸泡法提取百合多糖;在碱性条件下,百合多糖水溶液与三氯化铁反应合成LPC;用邻菲罗啉分光光度法和原子吸收光谱法测定LPC中铁(Ⅲ)的含量。结果:LPC是深棕红色无定型粉末,LPC中铁(Ⅲ)的含量:邻菲罗啉分光光度法测定结果为20.81%,原子吸收光谱法测定结果为20.10%。结论:邻菲罗啉分光光度法和原子吸收光谱法的测定结果基本一致。

Objective：Lily polysaccharide was extracted from lily,then lily polysaccharide-Fe（Ⅲ） complex（LPC） was synthesized with lily polysaccharide and FeCl 3 .We will establish the method of determination of iron in LPC.Method：Lily polysaccharide was abstracted with warm water;LPC was synthesized with lily polysaccharide and FeCl 3 under the alkaline condition;The determination of Fe（Ⅲ） was done with the method of ferroin-Fe（Ⅱ） and atomic absorption spectrophometry.Result：LPC was heavy brownish red and shapeless powder.Content of Fe（Ⅲ） at LPC was 20.81%（ferroin-Fe（Ⅱ） ） and 20.10%（atomic absorption） .Conclusion：The results are about the same.