摘要
目的:建立独一味制剂中山栀苷甲酯的HPLC测定方法,为进一步控制独一味制剂的质量提供良好的参考。方法:采用HPLC反向色谱柱Symmetry C18(4.6mm×150mm,5μm),梯度洗脱,0~9min乙腈-水(9∶91),9~20min乙腈-水(15∶85),流速1.0mL·min-1,柱温20℃,检测波长235nm。结果:山栀苷甲酯浓度在9.6~115.2μg·mL-1范围内与峰面积积分值线性关系良好;加样回收率为97.69%。从所有的制剂中均检测出山栀苷甲酯,但含量差别较大。结论:该方法准确、简便、专属性强、重现性好,适用于独一味制剂中山栀苷甲酯的含量测定。
Objective:To establish a HPLC method for the determination of shanzhiside methylester in the preparations of Lamiophlomis rotata.Method:HPLC analysis was performed on Symmetry C 18(5μm) column and gradient elution0-9 min,acetonitrile-water(9∶91) ,9-20 min,acetonitrile-water(15∶85) was used as the mobile phase at a flow rate of 1.0 ml·min^-1 .The column temperature was 20℃and the detection wavelength was at 235 nm.Result:The linear range of shanzhiside methylester was 9.6~115.2μg·mL^-1(r=0.999 8) and the average recovery was 97.69%.Shanzhiside methylester could be detected from all preparations and the content variedgreatly.Conclusion:The method can be applied to determine the content of shanzhiside methylester in the preparations of L.rotata.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第7期50-52,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
全军中医药研发推广项目(2006032001)
甘肃省科技攻关项目(2GS054-A43-014-14)