小鼠灌胃大枣提取物后血清和海马组织中cyclic AMP的含量变化

The Cyclic AMP Content with Time Variation after Oral Administration of the Extract of Fructus Jujubae in Mice

目的:观察小鼠ig给予大枣提取物(0.35g·kg-1)后24h内血清和海马组织中cyclicAMP(cAMP)含量随时间的变化规律,初步阐明大枣提取物抗抑郁作用可能的机制。方法:首先采用高效液相色谱法测定大枣提取物种cAMP的含量,再使用健康ICR小鼠,单次ig给予大枣提取物后在0.5,1,2,4,6、8,12,24h股动脉取血,分离海马组织,采用荧光ELISA法测定血清和海马组织中cAMP浓度。结果:大枣提取物中cAMP的平均含量为1.54%。血清中cAMP浓度在0.5h达最大值,在8h内逐渐减少,在8～12h又明显升高,此后又开始降低。而海马组织中cAMP浓度在前6h之内缓慢升高,6～8h内明显升高,24h达最大值。结论:大枣提取物中可能同时存在具有磷酸二酯酶抑制作用的物质,能够在6～12h内抑制磷酸二酯酶的活性,增加cAMP的浓度,这可能是大枣提取物抗抑郁的作用机制之一。

Cyclic AMP is one of the bioactive components in the extract of Fructus Jujubae,which is reported to be involved with the treatment of depression. To investigate the content of cAMP in the plasma and hippocampus according to the time variation after a single administration of the extract orally at the dose of 0. 35 g· kg^-1 in mice,the plasma and hippocampi were collected at the time of 0. 5,1,2,4,6,8,12 and 24 h and the ELISA method is used to determine the content of cAMP. As a result,the content of cAMP in plasma reaches to the maximum at the 0. 5 h,and then decreases gradually from the 0. 5 h to the 8 h. However,the content reversely increases rapidly from the 8 h to the 12 h,while decreases again in the following 12 hours. As to the hippocampi,differences are significant. The cAMP content in hippocampi is ascending gently in the first 6 hours and increasing quickly in the following hours. It is an indication that some bioactive substances in the extract of Fructus Jujubae are likely to be the specific inhibitor of Phosphodiesterase type Ⅳ（PDE4） . Therefore,the extract treated to the mice can inhibit the activity of PDE4 and increase the content of cAMP in the plasma and hippocampi during the time of 6 to 12 h. Thus,a possible mechanism for the antidepressant like effect of the extract of Fructus Jujubae is involvedwith the inhibition of PDE4.