双根清脑颗粒对血管性痴呆模型大鼠行为学及血清超氧化物岐化酶、丙二醛、一氧化氮、一氧化氮合酶的影响

Effects of Shuanggen Qinnao Granules on Animal Behavior and Superoxide Dismutase,Malondialdehyde,Nitric Oxide and Nitric Oxide Synthase in Serum of the Rat Model of Vascular Dementia

目的:观察双根清脑颗粒对血管性痴呆(VD)模型大鼠行为学,血清中超氧化物岐化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、一氧化氮合酶(NOS)的影响,探讨其干预VD的机制。方法:采用栓子注入法制成VD大鼠模型,给予双根清脑颗粒及脑复康ig15d,Morris水迷宫测定大鼠学习记忆能力,检测血清中的SOD,NOS活性及MDA,NO的含量。结果:与模型组比较,双根清脑颗粒组大鼠逃避潜伏期和首次穿越平台的时间明显缩短(P<0.05,P<0.01),中、低剂量组及脑复康组SOD活性升高,MDA含量降低(P<0.05),各用药组SOD/MDA的比值均升高(P<0.01)。各用药组血清NO含量降低(P<0.05,P<0.01),中、低剂量显著降低NOS活性(P<0.01)。结论:双根清脑颗粒可显著改善VD模型大鼠的学习记忆能力,提高血清SOD活力、降低血清MDA,NO的含量及NOS活性。

Objective：The purpose of this research is to explore the effects that Shuanggen Qingnao Granules towards vascular dementia（VD） by observing animal behavior and superoxide dismutase,malondialdehyde,nitric oxide and nitric oxide synthase levels in serum of VD rat model. Method：Vascular dementia（VD） rat models was made by injecting the same kinds of thrombotic emboli to the catheter of the external carotid artery. Morris water maze test is used for measuring the learning and memory ability. The photochemical reaction for contents of SOD in serum and MDA as well as NOS activity and nitratase method for NO levels were used. Result：The time for the rats taken Shuanggen Qingnao Granules to escape the latent period and to cross the platform for the first time was significantly shortened（P〈0. 05,P〈0. 01） . SOD activity increased in medium and low-dose rehabilitation groups. And the MDA content decreased. The ratio of SOD and MDA（SOD /MDA） in the treated groups has all elevated（P〈0. 01） . Besides,the NO contents in the treated groups decreased（P〈0. 05,P〈0. 01） . And the NOS activity in the medium and low-dose groups increased（P〈0. 01） . Conclusion：Shuanggen Qingnao Granules can significantly improve the learning and memory ability of the rats with VD. It can also increase SOD activity. Meanwhile,it de-creases MDA and NO contents as well as NOS activity.