昼夜给予逍遥散对肝损伤大鼠的保护作用

Protective Effects of Xiaoyao Powder Administrated in the Morning or Evening on Liver Injury in Rats

目的:研究昼夜不同时间给予逍遥散对CCl4诱发大鼠肝损伤保护作用的差异。方法:采用sc40%CCl4制备大鼠肝损伤模型,分别于早上8点和晚上8点ig逍遥散,连续12d,观测大鼠一般行为状态、体重、血清丙氨酸氨基转换酶(ALT)和天门冬氨酸氨基转换酶(AST)、肝组织超氧化物岐化酶(SOD)和丙二醛(MDA)以及肝组织病理学变化。结果:与模型组比较,逍遥散能显著改善CCl4诱导大鼠肝损伤模型的体重曲线和行为状态;显著降低血清中ALT,AST含量;升高肝组织SOD的活性,降低MDA的含量;减轻肝脏病理损伤程度。同时,多数指标显示晚间给药和低剂量组的效果更好。结论:逍遥散具有明显的保肝降酶作用,其疗效与给药时间密切相关。

Objective：To investigate the hepatoprotective effect and its circadian variations of Xiaoyao Powder and the possible mechanisms for protection on liver injury in rats. Method：The rat model of liver injury was induced by subcutaneous injection of 40% CCl4. The rats were given Xiaoyao Powder by gavage in the morning or evening for 12 days. General behavior state,body weight change,the levels of ALT,AST in serum and SOD and MDA activites in liver homogenate were detected. The pathology of liver was observed. Result：Compared with the model group,the Xiaoyao Powder was found to significantly improve the body weight and general behavior state,reduce the serum levels of ALT,AST and MDA,increase the activity of SOD,improve the pathological changes of liver tissue. Meanwhile,evening administration and the low dose was displayed a very good effect in most of the indexes. Conclusions：Xiaoyao Powder shows significant protective action on liver injury in rats. The effect of Xiaoyao Powder may be closely related to administration time.