羊栖菜多糖对2型糖尿病大鼠肾脏保护作用及其机制

Protective effects of Sargassum fusiforme polysaccharide on type 2 diabetes mellitus nephropathy rats and its mechanism

目的研究羊栖菜多糖(Sargassumfusi forme polysaccharide,SFPS)对2型糖尿病大鼠肾脏保护作用及其机制。方法将Wistar大鼠经链脲佐菌素造2型糖尿病大鼠模型,试验组给予SFPS 1.6,0.8和0.4 g.kg-1.d-1,灌胃治疗4 w。12 w后,观察肾功能、尿蛋白、肾脏病理变化。并以逆转录PCR检测肾脏转化生长因子β1(transforming growth factorβ1,TGF-β1)及母亲DPP同源物7(mothers against decapentaplegichomolog 7,Smad 7)的表达。结果 SFPS能显著改善肾脏功能。模型组大鼠肾脏TGF-β1和Smad 7 mRNA表达显著升高(P<0.01),而SFPS试验组TGF-β1和Smad 7 mRNA表达显著降低(P<0.01)。结论 SFPS对糖尿病大鼠肾组织有明显保护作用,其机制可能通过抑制肾脏TGF-β1和Smad 7 mRNA的表达。

Objective To study the protective effect and mechanism of SFPS on the kidney of type 2 diabetes mellitus rats.Methods Type 2 diabetes mellitus nephropathy model rats were established by STZ iv.and high sugar,lipid feeding,and SFPS were given ig.at doses of 1.6,0.8,0.4 g·kg-1 for 4 weeks.After 12 weeks,the renal function,urinary protein and the renal tissue were determined.The expressions of TGF-β1 and Smad 7 in renal tissue were detected by RT-PCR.Results SFPS could improve the renal function.The expressions of TGF-β1 and Smad 7 mRNA in the kindey were markedly up-regulated in diabetic model group rats,and the increased expressions were reduced by SFPS（P0.01）.Conclusion The renal protective effect of SFPS may be through inhibiting-expression of TGF-β1 and Smad 7 mRNA.