乳铁蛋白对大黄鱼仔鱼生长、存活和抗应激能力影响的研究

Effect of bovine lactoferrin on the growth,survival and stress resistance of Pseudosciaena crocea larvae

用不同水平（分别为T1组：0mg／L；T2组：50mg／L；T3组：100mg／L；T4组：150mg／L）乳铁蛋白（lactoferrin，简称LF）强化的卤虫（Artemia franciscana）幼体投喂9～23日龄大黄鱼（Pseudosciaena crocea）仔鱼。结果表明，摄食LF强化卤虫幼体的大黄鱼仔鱼其生长无明显改善（P〉0．05），但大黄鱼仔鱼的成活率和抗应激能力显著提高，T3（60％）和T4（80％）组的成活率显著高于T1（40％）和T2（30％）组（P〈0．05）。T4组大黄鱼仔鱼的耐干露能力最强，T4组和T3组的抗高盐度（65）应激能力明显的强于T1和T2组（P〈0．05），T4组和T3组之间无明显的差异（P〉0．05）。经4h高温（32℃）曝露后，T1和T2组的仔鱼全部死亡，T4组和T3组的成活率分别为30％和23．3％。即仔鱼的成活率和抗应激能力受卤虫强化LF水平的影响显著，LF强化的有效剂量为不低于100mg／L强化水体。

A feeding trial was conducted to investigate the effect ofArtemiafranciscana nauplii enriched with different levels of bovine lactoferrin （LF）（T1： 0mg/L; T2： 50mg/L; T3： 100mg/L; and T4： 150mg/L）on the growth and survival as well as stress resistance of the larvae of large yellow croaker Pseudosciaena crocea between 9 and 23 days after hatching. Dietary LF did not affect growth rate of larvae under the conditions in this study （P〉0.05）. At the termination of feeding trial, larvae fed Artemia nauplii enriched with 100 mg/L or 150 mg/L LF had signifi- cantly higher survival rate than those of the other treatments （P 〈 0.05）. Larvae fed Artemia nauplii enriched with 150 mg/L LF showed the lowest accumulated mortality after being exposed to the air for 5 min or to 32℃ seawater for 4 h. The larvae fed Artemia nauplii enriched with 100 mg/L or 150 mg/L LF showed longer survival time than those of the other groups （P〈0.05） after being exposed to high salinity seawater （65）. Results suggested that supplemental levels of LF markedly affected the survival and stress resistance of yellow croaker larvae. The supplemental levels of LF should be over 100 mg/L.