CXCL12／CXCR4生物轴在头颈部鳞癌淋巴转移的作用

The relationship between CXCL12/CXCR4 biological axis and neck lymphatic metastasis in head and neck squamous carcinoma

目的 观察趋化因子受体4（CXCR4）及其配体12（CXCL12）在头颈部鳞癌（HNSCC）组织及颈部淋巴结中的表达,分析CXCR4、CXCL12的表达与HNSCC临床病理关系,探讨CXCL12/CXCR4生物轴在HNSCC淋巴转移中的作用.方法 半定量逆转录.聚合酶链反应（RT-PCR法）及免疫组织化学SP法检测65例HNSCC组织、15例正常口腔组织及良性病变中CXCR4、CXCL12基因及蛋白表达.结果 RT-PCR结果：CXCR4在鳞癌转移组、非转移组、良性对照组中的表达分别为0.406±0.044、0.464±0.068、0.900±0.108,各组间表达的差异有统计学意义（P〈0.05）.转移组、未转移组淋巴结中CXCL12的表达分别为0.935±0.087、0.861±0.047,差异无统计学意义（P〉0.05）.免疫组织化学结果：CXCR4在鳞癌转移组、非转移组、良性对照组中的表达率分别为71.4%、33.3%、13.3%.各组间表达的差异有统计学意义（X2=65.23,P〈0.05）.CXCR4的表达与临床分期、肿瘤分化程度、淋巴结转移显著相关（P〈0.01）,与年龄、性别无明显相关（P〉0.05）.淋巴结中高表达CXCL12,CXCL12的表达在所有参数的分组表达中差异均无统计学意义（P〉0.05）.结论 CXCR4的高表达可能赋予HNSCC细胞较强的局部侵犯及淋巴转移潜能 CXCL12/CXCR4生物轴在HNSCC淋巴转移中可能发挥作用.

Objective To analyze the relationship between the expression of CXCR4 and CXCL12 in head and neck squamous carcinoma（HNSCC） and clinicopathological features of HNSCC, and explore the effect of CXCR4/CXCL12 biological axis on lymph node metastasis in HNSCC. Methods The clinicopathological data of 65 patients with HNSCC undergoing surgical resection were collected and reviewed. Fifteen cases of normal mucosa and benign lesion served as controls. The differential expression of CXCR4 and CXCL12 in different groups was detected by using immunohistochemical staining and reverse transcrip-tion-polymerase chain reaction（RT-PCR） to identify their relationship with lymph node metastasis. Results The expression of CXCR4 mRNA was 0.406 ± 0. 044, 0.464 ± 0. 068 and 0. 900 ± 0. 108 in lymp metastasis, non-lymph metastasis and control groups respectively. There was significant difference in the CXCR4 mRNA expression between HNSCC group and control group （P〈0.05）. The CXCL12 mRNA expression was 0. 935 ± 0.087 and 0. 861±0.047 in lymp metastasis and non-metastasis groups （P〉0.05 ）. The statistical analysis revealed that the expression rate of CSCR4 protein was 71.4% （25/35）, 33. 3% （10/30） and 13.3% （2/15） in lymph metastasis, non-metastasis and control groups respectively. Over-expression of CXCR4 was significantly correlated with clinical stage, depth of invasion and lymph node metastasis （P〈0.01）. There was no significant difference in the CXCL12 expression among the groups. Conclusion Overexpression of CXCR4 was related to lymph node metastasis and classification in HNSCC. CXCL12/CXCR4 biological axis might promote orientation metastasis to cervical lymph node in HNSCC.