肉桂对慢性脑缺血大鼠氧化应激及神经因子表达的影响

Effect of Cinnamon Intervention on Oxidative Stress and Nerve Factor Expression in Rats with Chronic Cerebral Ischemia

目的探讨肉桂水提液对慢性脑缺血大鼠的脑保护作用。方法选用雄性Wistar大鼠,双侧颈总动脉永久结扎复制慢性脑缺血模型,成模后随机分为模型组和肉桂高、低剂量组,每组20只,并设置20只假手术大鼠作对照。肉桂高、低剂量组于术后24h分别给予肉桂水提液以4.2g/kg、2.1g/kg剂量灌胃,连续4周。4周后用Y型迷宫检测认知能力及脑组织超氧化物歧化酶(SOD)活性及丙二醛(MDA)的含量、神经生长因子(NGF)、脑原性神经生长因子(BDNF)表达。结果与假手术组相比,模型组认知能力下降,脑组织SOD活性下降,MDA的含量增多。肉桂能提高SOD活性,减少MDA含量,提高NGF、BDNF表达,且高剂量组优于低剂量组。结论肉桂通过提高脑组织SOD活性及NGF、BDNF表达,降低MDA含量发挥其脑保护作用。

Objective To investigate the brain protection effect of cinnamon water extract on chronic cerebral ischemia rats. Methods We selected male Wistar rats to make chronic cerebral ischemia model by bilateral carotid artery ligation permanently,and the models were randomized into a model group,cinnamon high or low dose group,with 20 in each group. A control group with 20 rats was treated with sham operation. The cinnamon high and low dose groups were given cinnamon water extract 4.2g/kg and 2.1g/kg respectively by gavage in succession for 4 weeks. Y-maze test was used to test the cognitive ability of the rats. The superoxide dismutase （SOD） activity,malondialdehyde （MDA） content,and the expressions of nerve growth factor （NGF） and brain-derived neurotrophic factor （BDNF） were determined. Results Compared with the sham-operated group,the cognitive ability of the model group declined,the SOD activity of brain tissues decreased,and the MDA content increased. Cinnamon improved the SOD activity,reduced the MDA content,increased the NGF and BDNF expressions,and the effect of high-dose group was better than that of the low-dose group. Conclusion Cinnamon plays its role in cerebral protection through increasing the SOD activity and expression of NGF and BDNF and reducing content of MDA.