补肺化痰方对肺虚痰阻证模型大鼠Na~＋-K~＋-ATP酶活性的影响

Effect of Bufei Huatan Formula on the Activity of Na^+-K^+-ATPase in Rat Models with Phlegm Retention due to Lung Deficiency

目的探讨肺虚痰阻证模型大鼠Na+-K+-ATP酶活性变化及补肺化痰方的干预效果。方法 30只SD大鼠随机分为正常组、模型组和治疗组各10只,采用冷风刺激加烟熏法致大鼠肺虚痰阻证模型,治疗组给予补肺化痰方,正常组和模型组给予等体积生理盐水,共14天。观察各组大鼠一般活动情况、浓氨水刺激后3min内咳嗽次数、大鼠肺组织病理改变及Na+-K+-ATP酶活性变化。结果用浓氨水刺激诱发咳嗽后,3min内模型组及治疗组咳嗽次数较正常组增加(P<0.05);治疗组大鼠病理切片示气管、支气管杯状细胞增生,腺体肥大、增生均较模型组减轻;模型组和治疗组的Na+-K+-ATP酶活性均低于正常组(P<0.05),但治疗组明显高于模型组(P<0.05)。结论补肺化痰方可上调肺虚痰阻证模型大鼠Na+-K+-ATP酶活性,肺泡膜上Na+-K+-ATP酶的活性降低可能是产生肺虚痰阻证的机制之一。

Objective To study the effect of Bufei Huatan Formula （Herbal formula for reinforcing the lung to dissolve phlegm） on the changes of activity of Na＋-K＋-ATPase of the rat models with phlegm retention due to lung deficiency. Methods Thirty SD rats were randomized into normal group,model group and treatment group with 10 in each. The models of lung deficiency with phlegm retention were made with cold wind and smoking. The treatment group received Bufei Huatan Formula,the normal group and model group were given normal saline of equal-volume for 14 days. General activities,times of cough induced by the stimulation of concentrated ammonia within 3 minutes,pathological changes in the lung tissues,and changes of activity of Na＋-K＋-ATPase were observed. Results The times of cough of the model and treatment groups were increased compared to that of the normal group （P0.05）. The pathological section of the treatment group showed that the goblet cell hyperplasia and glandular hypertrophy of trachea and bronchi were milder than those of the model group. The activity of Na＋-K＋-ATPase of the model and treatment group was lower than that of the normal group （P0.05）,but the treatment group was significantly higher than the model group （P0.05）. Conclusion Bufei Huatan Formula is able to up-regulate the activity of Na＋-K＋-ATPase of the model rats with phlegm retention due to lung deficiency. The decrease of activity of Na＋-K＋-ATPase of the alveolar wall basement membrane is possibly one of the important mechanisms inducing the lung deficiency with phlegm retention.