药用淫羊藿品种的分子鉴定研究——朝鲜淫羊藿的位点特异性PCR鉴定

Molecular authentication of medicinal Epimedium species——A case of diagnostic PCR authentication of Epimedium koreanum

目的:建立朝鲜淫羊藿的位点特异性PCR鉴定方法,用于朝鲜淫羊藿来源鉴定和药材质量控制。方法:依据自己测得和来自于GenBank主要药用淫羊藿种的核糖体基因组ITS序列,比对分析寻找朝鲜淫羊藿区别于其他种的变异位点,并根据这些变异位点设计专属性的PCR鉴定引物对植物样本进行扩增,并用药材饮片进行验证。结果:本研究获得了2对朝鲜淫羊藿特异性鉴定引物,能够区别朝鲜淫羊藿与其他药用品种,本方法适用于淫羊藿药材和饮片。

Objective： To establish the allelespecific diagnostic PCR methods for Epimedium koreanum.Method： ITS of eight medicinal Epimedium species were sequenced and downloaded from GenBank,and aligned to find the specific sites in E.koreanum.The diagnostic primers were designed based on the sites,and used to amplify all the samples,then verified with the crude drugs and decoction pieces from market.Result and Conclusion： Two pairs of allele-specific diagnostic primers were successfully used to indentify E.koreanum from other medicinal species,and also can be used to identify the crude drugs and decoction pieces.