宝肾方对糖尿病大鼠肾组织CTGF和MMP-9的影响

Effects of Shenbao recipe on expressions of CTGF and MMP-9 in diabetic nephropathy rats

目的:探讨宝肾方对糖尿病大鼠的治疗作用及其对肾组织结缔组织生长因子(CTGF)和基质金属蛋白酶-9(MMP-9)表达的影响。方法:以链脲佐菌素(STZ)诱导建立糖尿病大鼠模型。随机分为对照组(C组)、糖尿病组(D组)和糖尿病宝肾方治疗组(DB组)。给药8周后检测各组生化指标,观察肾组织病理形态学改变,采用免疫组化方法分析肾组织CT-GF和MMP-9蛋白的表达情况。结果:和对照组相比,糖尿病组肾组织CTGF蛋白表达明显升高[(0.234±0.009)vs(0.146±0.007),P(0.01],而MMP-9蛋白表达明显降低[(0.146±0.006)vs(0.236±0.007),P(0.01],肾脏肥大指数、24 h尿蛋白、血糖、胆固醇、血肌酐明显升高(P(0.01);和糖尿病组相比,宝肾方治疗组肾组织CTGF蛋白表达降低[(0.194±0.008)vs(0.234±0.009),P(0.01],而MMP-9蛋白表达升高[(0.175±0.007)vs(0.146±0.006),P(0.01],肾脏肥大指数降低(P(0.05),24 h尿蛋白、胆固醇、血肌酐降低(P(0.01)。结论:宝肾方对糖尿病大鼠肾脏具有保护作用,其机制可能与影响肾组织CTGF和MMP-9的表达有关。

Objective： To investigate the effects of Shenbao recipe on expressions of CTGF and MMP-9 in the kidney of diabetic rats.Method： Wistar rats were randomized divided into control group（group C）,diabetic rat group（group D） and Bao′shen recipe treatment group（group DB）.Streptozotocin（STZ） was injected through abdominal cavity to make models of diabetic rats.Administration for 8 weeks,renal function and pathological changes of rats′ renal tissue were observed,protein expression of CTGF and MMP-9 in renal tissue was investigated by immunohistochemistry analysis.Result： Compared with group C,expressions of CTGF in renal tissue of group D were higher [（0.234±0.009） vs（0.146±0.007）,P0.01],while the expressions of MMP-9 were lower [（0.146±0.006） vs（0.236±0.007）,P0.01].Kidney hypertrophy index,24-hour urinary protein,blood glucose,cholesterol and serum creatinine were increased significantly in the group D.The level of MMP-9 expression was increased [（0.175±0.007） vs（0.146±0.006）,P0.01],while the level of CTGF expression was reduced [（0.194±0.008） vs（0.234±0.009）,P0.01] by using Shenbao recipe.In group DB,all index including kidney hypertrophy index,24-hour urinary protein,cholesterol and serum creatinine were obviously lower than diabetic rat group.Conclusion： Shenbao recipe can depress the expressions of CTGF and increase the expressions of MMP-9 in the kidney of diabetic rats,which can be served as a protective effect to prevent progress of diabetic nephropathy.