广金钱草及其制剂的HPLC指纹图谱研究

Study on HPLC Fingerprint of Desmodium styracifolium and Its Preparations

目的:建立广金钱草及其制剂的高效液相色谱(HPLC)指纹图谱,为其质量评价提供依据。方法:色谱柱为迪马ODS-C18(250mm×4.6mm,5μm),流动相为甲醇-2%磷酸溶液(梯度洗脱),流速为1.0mL·min-1,柱温为25℃,检测波长为360nm,记录广金钱草及其制剂的HPLC指纹图谱。结果:广金钱草药材和制剂均有13个共有峰,多数峰可以达到较好分离。结论:本方法具有良好的精密度、稳定性和重复性,建立的指纹图谱检测标准可以作为广金钱草及其制剂质量评价的主要依据之一。

OBJECTIVE：To establish RP-HPLC fingerprints of Desmodium styracifolium and its preparations in order to provide basis for the quality evaluation of them.METHODS：The separation was performed on ODS-C18（250 mm×4.6 mm,5 μm） column with mobile phase consisted of methanol-20% phosphoric acid（gradient elution）.The detection wavelength was set at 360 nm and flow rate was 1.0 mL·min^-1.Column temperature was set at 25 ℃.The RP-HPLC fingerprint of D.styracifolium and its preparations were recorded.RESULTS：There were 13 common peak in RP-HPLC fingerprint of D.styracifolium and its preparations.CONCLUSION：The method is accurate,stable and reproducible for basis of quality evaluation and RP-HPLC finger print of D.styracifolium and its preparations.