对2005年版《中国药典》小儿热速清口服液中含量测定方法的改进

Improvement of Determination Method of Xiao'er Resuqing Oral Solution in Chinese Pharmacopiea(2005 edition)

目的:改进2005年版《中国药典》小儿热速清口服液中含量测定的方法。方法:采用高效液相色谱法测定制剂中黄芩苷含量,色谱柱为Agilent zorbax SB-C18(150mm×4.6mm,5μm),流动相为甲醇-水-磷酸(45:55:0.2),流速为1.0mL·min-1,检测波长为276nm。结果:黄芩苷进样浓度在0.5～100μg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均回收率为99.83%,RSD=0.99%(n=9)。结论:本方法快速、简便、准确,可为新版《中国药典》中小儿热速清口服液中含量测定方法提供参考依据。

OBJECTIVE：To improve the determination method of Xiao＇er resuqing oral solution in Chinese Pharmacopeia（2005 edition）.METHODS：HPLC method was used to determine the content of baicalin.The separation was performed on Agilent zorbax SB-C18（150 mm×4.6 mm,5 ？m） column with methanol-water-phosphoric acid（45：55：0.2） as the mobile phase at flow rate of 1.0 mL·min^-1.The detection wavelength was set at 276 nm.RESULTS：The linear range of baicalin was 0.5～100 μg·mL^-1（r=0.999 9） with an average recovery of 99.83%（RSD=0.99%,n=9）.CONCLUSION：The method is rapid,convenient and accurate,and can be used as reference evidence for the content determination of Xiao＇er resuqing oral solution in Chinese Pharmacopoeia（2005 edition）.