利用TCRVβ家族CDR3长度分析检测HCL和c-ALL的克隆性增殖T细胞

ANALYSIS OF T CELL CLONALITY BY CDR3 SIZE OF T CELL ANTIGEN RECEPTOR Vβ REPERTOIRE IN HCL AND C ALL

目的推介检测分析Ｔ细胞克隆性增殖的新方法，探讨判别恶性肿瘤免疫状态的新途经。方法应用ＲＴ－ＰＣＲ检测Ｔ细胞受体（ＴＣＲ）Ｖβ２４个亚家族的互补决定区３（ＣＤＲ３）长度，并经基因扫描（ｇｅｎｅｓｃａｎ）分析检测３例多毛细胞白血病（ＨＣＬ）和１例普通型急性淋巴细胞白血病（ｃ－ＡＬＬ）病人外周血的Ｔ细胞克隆性。结果４例病人的部分Ｖβ家族的扩增产物出现单峰（单克隆）或—主峰（寡克隆）图像，而正常对照的全部ＰＣＲ产物呈多峰（多克隆）图像。结论ＨＣＬ和ｃ－ＡＬＬ病人外周血中存在克隆性生殖Ｔ细胞，可能是宿主对白血病抗原的一种直接反应。

Objective To Analyse of T Cell clonality by CDR3 sige of T cell antigen receptor Vβ repertoire in HCL and C ALL.Methods Peripheral blood mononuclear cell samples from 3 cases of HCL and 1 case of c ALL were investigated for CDR3(conplementarity determining region 3) size analysis of T cell receptor Vβ repertoir using reverse transcriptase polymerase chain reaction(RT CPR). The products were further analyzed by genescan to identify T cell clonality. Results The results showed that some Vβ subfamily PCR products from 4 patients comtained monopeak(monoclone) or a dominant peak(oligoclone). In contrast multipeak(polyclone) distributions were found in all Vβ subfamily PCR products from normal control cases. Conclusion T cell clonal expansion may be found in HCL and c ALL cases that may indicate a host respones directed against leukemia related antigen. In addition, it may be useful to detect the minimal residual disease.