急性非淋巴细胞白血病降钙素基因甲基化的检测及其意义

DETECTION AND ITS SIGNIFICANCE OF THE HYPERMETHYLATION OF CALCITONIN GENE IN ACUTE NONLYMPHOBLASTIC LEUKEMIA

目的探讨急性非淋巴细胞白血病（ＡＮＬＬ）中降钙素基因（ＣＴ基因）的甲基化状况及其意义。方法利用限制性内切酶和聚合酶链反应（ＰＣＲ）技术。结果２５例ＡＮＬＬ患者中１８例检测到ＣＴ基因高度甲基化，阳性率７２％，而且实验的敏感性至少达到１０－３水平，结论ＣＴ基因高度甲基化较普遍地存在于ＡＮＬＬ，可作为ＡＮ－ＬＬ恶性细胞的分子标志，用于ＡＮＬＬ患者治疗后微小残留病（ＭＲＤ）的检测，以利于预测复发、指导治疗和评价疗效。

Objective To study the methylation pattern of calcitonin gene (CT gene) in acute nonlymphoblastic leukemia(ANLL)and ist implication. Methods By using restriction endonuclease and polymerase chain reaction(PCR) techniques. Results The positive rate was 72%.And the sensitivity of this method was at least 3 logs (1 in 1 000).Conclusion This findings indicated that the hypermethlation of CT gene was common in ANLL.It might be used as a molecular marker for the tumor cells in ANLL. So that,it can serve as a useful tool for detection of minimal residual disease(MRD) in ANLL to predict early relapase, to guide the treatment and to evaluate the effectiveness of therapy.