幽门螺杆菌空泡毒作用

WT5”HZ〗Vacuolation induced by Helicobacter pylori

目的研究幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ，Ｈｐ）空泡毒作用。方法用体外细胞毒试验，结合细胞化学及荧光染色、透射电镜技术研究Ｈｐ空泡毒作用及其病理机制。结果７８．２６％的Ｈｐ相关消化性溃疡患者感染的是空泡毒作用阳性Ｈｐ（Ｔｏｘｉｎ＋），而只有４２．８６％的胃炎患者感染的是Ｈｐ（Ｔｏｘｉｎ＋）。Ｈｐ（Ｔｏｘｉｎ＋）引起的细胞空泡样变，吖啶橙及酸性磷酸酶染色阳性；透射电镜显示空泡为双层膜结构。结论Ｈｐ（Ｔｏｘｉｎ＋）感染与Ｈｐ相关消化性溃疡密切相关。Ｈｐ（Ｔｏｘｉｎ＋）引起的空泡是自噬体，是细胞在毒性物质作用下表现的病理现象。

Objective The vacuolating effect by Helicobacter pylori(Hp) was investigated. Methods Cytotoxic test in vitro combined with cytochemical stain、fluorescent stain、transmission electronmicroscopy were used to investigate the vacuolating effect by toxin of Hp and its pathogenetic mechanism. Results 78.26% patients with peptic ulcer and 42.86% patients with gastritis were infected with Hp(toxin+). It was positive in vacuoles with acridine orange and acid phosphatase stain. Transmission electronmicroscopy revealed presence of abounding membrane.Conclusion There was closed relations relationship between infection with Hp(toxin+) and peptic ulcer disease. The vacuolation induced by Hp(toxin+) was autophagosome, which was the pathological manifestation induced by the toxin.