肿瘤坏死因子α多克隆抗体对新生大鼠缺氧缺血性脑损伤的保护作用研究

The protective effects of polyclonal antibody to tumor necrosis factorα on hypoxic ischemic brain damage (HIBD) of neonatal rats

目的研究肿瘤坏死因子（ＴＮＦ）α多克隆抗体对新生大鼠缺氧缺血性脑损伤（ＨＩＢＤ）后大脑的保护效应。方法制备左脑ＨＩＢＤ的新生大鼠模型，于ＨＩＢＤ前后分别腹腔注射一定浓度的ＴＮＦα多克隆抗体，观察用药前后损伤大脑脑水含量及组织病理学变化。结果ＨＩＢＤ前后腹腔注射ＴＮＦα多克隆抗体可明显降低脑水含量（Ｐ＜０．０１），大脑水肿明显减轻，神经元死亡明显减少，但对其相应ｍＲＮＡ转录无影响。结论ＴＮＦα多克隆抗体可以拮抗ＴＮＦα的炎性作用。

Objective To study the protective effects of polyclonal antibody to tumor necrosis factor(TNF)α on hypoxic ischemic brain damage (HIBD) of neonatal rats. MethodsWe made a model of left brain HIBD in neonatal rats and injected TNFα polyclonal antibody into abdominal cavity before and after HIBD. Brain water content and histopathology were observed. Results Polyclonal antibody of TNFα administered before or after HIBD decreased brain water content significantly ( P <0.01), but had no effects on corresponding mRNA expression. TNFα polyclonal antibody also attenuated edema of brain and death of neurons pathologically. Conclusions TNFα polyclonal antibody may antagonize the inflammatory function of TNFα and protect brain after HIBD.