单纯疱疹病毒脑炎早期诊断方法的研究

The study of the methods for early diagnosis of herpes simplex encephalitis

】目的探讨单纯疱疹病毒脑炎（ＨＳＥ）早期诊断的最佳方法。方法运用免疫细胞化学（ＩＣＣ）、原位杂交（ＩＳＨ）和聚合酶链式反应（ＰＣＲ）三种方法分别对２６例病理诊断为病毒性脑炎患者的脑组织标本进行检测单纯疱疹病毒（ＨＳＶ）。结果３例标本用ＩＣＣ检测出ＨＳＶ抗原，４例用ＩＳＨ、５例用ＰＣＲ检测出ＨＳＶ核酸，共６例被确诊为ＨＳＥ。结论３种方法均为早期诊断ＨＳＥ的敏感方法，尤以ＰＣＲ最敏感。

Objective To investigate the best early diagnostic method for herpes simplex encephalitis(HSE). Methods Paraffin embedded brain tissues of 26 patients with viral encephalitis by pathological diagnosis were studied by immunocytochemistry(ICC), in situ hybridization(ISH) and polymerase chain reaction(PCR) for the detection of herpes simplex virus(HSV). Results HSV antigen was detected in 3 cases by ICC , HSV nucleic acid was detected in 4 cases by ISH and in 5 cases by PCR,6 cases were diagnosed as HSE altogether. Conclusion Three methods were all sensitive methods for early diagnosis of HSE, in which PCR was the most sensitive.The combination of ICC and PCR was the best method for early diagnosis of HSE.