钙黄绿素荧光猝灭法测定辛硫磷

Fluorescence Quenching Method for the Determination of Phoxim by Calcein

中性介质中,生物小分子钙黄绿素与阳离子表面活性剂CTMAB缔合,体系荧光强度降低,而当加入辛硫磷后荧光强度继续降低并且降低的程度与加入辛硫磷的量在一定浓度范围内成线性.据此建立了一种测定辛硫磷的新方法,该法的线性范围为0～5.6mg/L,检测限为0.039mg/L.

In the presence of appropriate amounts of cationic surfactant of cetyl trimethyl ammonium bromide（CTMAB）,the anionic dye calcein combind with it and the fluorescence intensity of calcein-CTMAB can be decreased obviously by adding phoxim in the neutral medium.The decreased fluorescence intensity is proportional to the concentration of phoxim in rang of 0-5.6 mg/L and the detection limit is 0.039 mg/L.