摘要
建立一种快速、准确测定白鲜皮中梣酮含量的HPLC—MSMS分析方法。测定方法为：液相色谱条件为XDB—C18柱，流动相为甲醇-水，m（甲醇）：m（水）=65：35，流量为0．3mL／min。质谱条件为Fragmento电压100V，定量离子m／z215，碰撞能量4V；定性离子m／z187，碰撞能量10V。结果表明，质量浓度为0．0084～0．840mg／L，相关性R2=0．99999，回收率92．8％-100．0％；定性检出浓度（S/N=3）0．002mg／L；定量检出浓度（S／N=10）0．007mg／L。该方法准确可靠，适用于白鲜皮中梣酮的定量分析。
An analyzing method based on HPLC-MSMS was established to determine the fraxinellone content in Dictamnus dasycarpus Turcz. fleetly and truly. Methods： The condition of liquid chromatogram： XDB-C18 column, mobile phase： methanol ： water=65 ： 35, flow rate： 0.3 mL/min. The condition of mass spectrum： Fragmento voltage 110 V, quantificational ion m/z 215, collision energy 4 V; qualitative ion m/z 187, collision energy 10 V. Results： When the range of concentration was from 0.008 4 mg/L to 0.840 mg/L, relativity R2=0.999 99, the recoveries of this method in the range of 92.8%~100.0%. Qualitative concentration of detection （S/N=3） ： 0.002 mg/L, quantificational concentration of detection （S/N=3） ： 0.007 mg/L. Conclusion： The method was accurate. It can be applied to the determination of the fraxinellone content in Dictamnus dasycarpus Turcz patulin.
出处
《农产品加工（下）》
2010年第7期96-97,共2页
Farm Products Processing
关键词
白鲜皮
HPLC—MSMS
梣酮
Dictamnus dasycarpus Turcz
HPLC-MSMS
fraxinellone
