上调白血病骨髓基质细胞GJIC功能对Jurkat细胞药物敏感性的影响

Effect of up-regulation of GJIC function between leukemic bone marrow stromal cells on the sensitivity to chemotherapeutics of Jurkat cells

目的观察上调白血病骨髓基质细胞间隙连接细胞间通讯(GJIC)功能对共培养的人急性淋巴细胞白血病细胞株(Jurkat)细胞药物敏感性的影响。方法体外培养白血病骨髓基质细胞,转染Cx43基因,罗氏黄染料传输法检测转染后骨髓基质细胞的GJIC功能变化;将Jurkat细胞与转染Cx43基因的骨髓基质细胞共培养,观察共培养的Jurkat细胞对化疗药物甲氨蝶呤(MTX)药物敏感性的改变。结果转染Cx43基因的白血病骨髓基质细胞的GJIC功能明显增加;与转染Cx43基因的骨髓基质细胞共培养的Jurkat细胞在MTX作用下的存活率(61.3±3.24)%较未转染Cx43基因的骨髓基质细胞共培养的Jurkat细胞(82.4±3.12)%明显下降;与转染Cx43基因的骨髓基质细胞共培养的Jurkat细胞在MTX作用下的凋亡率(48.1±3.78)%较与未转染Cx43基因的骨髓基质细胞共培养的Jurkat细胞(18.5±3.43)%明显增加,差异有统计学意义(P<0.01)。结论上调白血病骨髓基质细胞GJIC功能能够增加共培养的Jurkat细胞对化疗药物的敏感性。

Objective To observe the effect of up-regulation of GJIC function between leukemic bone marrow stromal cells（BMSCs） on the sensitivity to chemotherapeutics of co-cultured Jurkat cells.Methods Cultured leukemic BMSCs in vitro,transfected Cx43 on BMSCs,and detected the GJIC function between BMSCs by using LY transmission assay.We then Observed the sensitivity to MTX of co-cultured Jurkat cells.Results The GJIC function of leukemic BMSCs which was transfected Cx43 were significant increased.The survival rate of Jurkat cells co-cultured with leukemic BMSCs transfected Cx43 was（61.3±3.24）%,which was significant decreased compared with Jurkat cell co-cultured with un-transfected Cx43 BMSCs（82.4±3.12）%.Meanwhile,the apoptotic rate of Jurkat cells co-cultured with leukemic BMSCs transfected Cx43 was（48.1±3.78）%,which was significant increased compared with Jurkat cell co-cultured with un-transfected Cx43 BMSCs（18.5±3.43）%,P0.01.Conclusion Up-regulation of GJIC function between leukemic BMSCs increased the sensitivity to chemotherapeutics of co-cultured Jurkat cells.