摘要
在pH10.23的Tris-HCl缓冲溶液中,阴离子主体小分子对羟基萘酚兰二钠盐(HNB)对阳离子表面活性剂十六烷基三甲基溴化铵(CTMAB)与脱氧核糖核酸(DNA:fsDNA、ctDNA)的共振光散射光谱有协同增强作用.考察了共振光散射(RLS)光谱的影响因素,在优化条件下共振光散射强度增加值(ΔI)与DNA浓度之间呈良好的关系,据此建立了一种测定DNA的新方法,该方法具有反应速度快(只须1min)、稳定性好、灵敏度高等特点.对于fsDNA、ctDNA的线性范围分别为0.05～2.5 mg/L,0.04～2.0 mg/L,检出限分别为25.9 ng/mL,24.9 ng/mL.用于fsDNA合成样测定效果较好.
A new and simple method for determination of Deoxyribonucleic acid was established.Which base on Deoxyribonucleic acids and CTMAB enhance the Resonance Light-Scattering（RLS） intensity in existing Hydroxy NaphtholL Blue Disodium Salt.In the worth of pH10.23 Buffer-solution of Tris-HCl,the relationships between the enhancement of RLS intensity and the concentration of two kinds of Deoxyribonucleic acids were studied,Under the optimal conditions.The linear rang are 0.05-2.5 mg/L,0.04-2.0 mg/L and The detection limits are 25.93 ng/mL,24.91 ng/mL for the fsDNA,ctDNA respectively.The method successfully applied to determination of three samples of synthesis and the results were satisfied.
出处
《湖南科技大学学报（自然科学版）》
CAS
北大核心
2010年第2期114-116,121,共4页
Journal of Hunan University of Science And Technology：Natural Science Edition
基金
湖南省自然科学基金资助项目(09JJ6021)
