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蓝隐藻的培养及色素-蛋白复合物分离 被引量：2

Culture and Pigment-Protein Complex Isolation of Chroomonas placiodea
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摘要 探索了蓝隐藻的实验室自行培养条件;改进了完整类囊体膜的制备方法,获得了状态较为理想的类囊体膜,其Chla/c比值可稳定在4左右.此外,建立并优化了色素-蛋白复合物的增溶以及蔗糖密度梯度离心分离的条件,得到了4种活性的色素-蛋白复合物,经光氧化活性测定,初步判定为捕光复合物Ⅰ、捕光复合物Ⅱ以及PSⅡ颗粒和PSⅠ颗粒.实验结果为今后进一步研究蓝隐藻光合系统结构奠定了基础. The cultural conditions of Chroomonas placiodea in laboratory are investigated,and the preparation of intact thylakoid is improved by which thylakoids in well conditions are obtained with Chl a/c ratios steady around 4.0.Moreover,the method of detergent resolution, along with the isolation by sucrose gradient centrifugation of pigment-protein complexes are established and optimized.4 active pigment-protein complexes are resolved which are designated as light-harvest complex I,light-harvest complex II,PS I and PS II pellets respectively according to the results of photooxidative activity assay.The present work will lay a solid foundation for further structural study of photosystems of C.placiodea.
作者 王扬 陈敏
机构地区 烟台大学化学生物理工学院
出处 《烟台大学学报（自然科学与工程版）》 CAS 北大核心 2010年第3期188-193,共6页 Journal of Yantai University(Natural Science and Engineering Edition)
基金 山东省优秀中青年科学家奖励基金资助项目(2006BS02016) 国家自然科学基金资助项目(40976083)
关键词 蓝隐藻 类囊体膜 色素-蛋白复合物 蔗糖密度梯度离心 C.placiodea thylakoid pigment-protein complex sucrose gradient centrifugation
分类号 Q946 [生物学—植物学]
  • 相关文献

参考文献12

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二级参考文献14

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共引文献37

同被引文献11

  • 1陈敏,孙力,李瑗.蓝隐藻色素蛋白复合物的分离和分析[J].烟台大学学报（自然科学与工程版）,2006,19(3):187-192. 被引量：3
  • 2Gantt E. Phycobiliproteins of Cryptophyceae [ M ] // Levandowsky M, Huthner S H. Biochemistry and Physiology of Protozoa. New York: Academic Press, 1979 : 121-137.
  • 3Glazer A N,Hixson J C S. Characterizeation of R-phy- cocyanin Chormophore content of R-phycocyanin and C-phy- coerythin [ J ]. Biol Chem, 1975,250:5487-5495.
  • 4Ludwig M, Gibbs S P. Localization of phycoerythrin at the lumennal surface of the thylakoid membrance in Rhodo- monas lens [J]. The Journal of Cell Biology,1989, 108:875 -884.
  • 5Lichtle C, Duval J C, Lemoine Y. Comparative bio- chemical, functional and ultrastructural studies of photosys- tern particles from a Cryptophycea: Cryptomonas rufescens; i- solation of an active phycoerythrin particle [ J ]. Biochimica et Biophysica Acta, 1987, 894( 1 ) : 76-90.
  • 6Vernon T O I, Glazer A N. Fluorescent phycobilipro- tein conjugatesfor analyses of cells and molecules[J ]. Cell Biol, 1982,93 ( 3 ) :981.
  • 7Laemmli U. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J].Nature, 1970, 227:680-685.
  • 8Min Chen Si Hui Li Li Sun.A novel phycocyanin-Chla/c_2-protein complex isolated from chloroplasts of Chroomonas placoidea[J].Chinese Chemical Letters,2007,18(11):1374-1378. 被引量：7
  • 9Sami Kere?che,Roman Kou?il,Gert T. Oostergetel,Fabrizia Fusetti,Egbert J. Boekema,Alexander B. Doust,Chantal D. van der Weij-de Wit,Jan P. Dekker.Association of chlorophyll a / c 2 complexes to photosystem I and photosystem II in the cryptophyte Rhodomonas CS24[J].BBA - Bioenergetics.2008(9)
  • 10Hiroto Kikuchi,Hiroshi Wako,Kei Yura,Mitiko Go,Mamoru Mimuro.Significance of a Two-Domain Structure in Subunits of Phycobiliproteins Revealed by the Normal Mode Analysis[J].Biophysical Journal.2000(3)

引证文献2

二级引证文献18

烟台大学学报（自然科学与工程版）
2010年 第3期

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