PCR方法检测淋巴系肿瘤的基因标志(附67例分析)

Detection of Gene Rearrangement in 67 Patients with Lymphoproliferative Disorders Using Polymerase Chain Reaction

应用多聚酶链反应（ＰＣＲ）方法检测６７例淋巴系肿瘤的基因标志，以５例正常人和５例急性非淋巴细胞白血病（ＡＮＬＬ）为对照。结果表明，ＩｇＨ基因重排只见于Ｂ细胞性肿瘤，具有Ｂ系特异性；ＴＣＲγ重排不仅见于Ｔ细胞，也可见于Ｂ细胞；５例ＵＡＬＬ中４例检出ＴＣＲγ重排。

Using polymerase chain reaction (PCR), we studied gene rearrangement in 67 patients with lymphoproliferative disorders, acute nonlymphoblastic leukemia (ANLL) and normals as control. The results demonstrated that IgH gene rearrangent was found in patients of B lymphoproliferative disorders only, TCR γ rearrangement was not only in T cell but in B cell clones. TCR γ rearrangement were detected in 4 of 5 patients U ALL which indicated that they have a higher tendency to develop T cell clones. Gene rearrangement is more sensitive than immunologic method in determination clones.