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乳酸脱氢酶同工酶的提取及活力测定 被引量:4

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摘要 以兔肌为实验材料,常规处理组织匀浆,差速离心,取微量上清液经稀释后为酶样品。以丙酮酸、还原型尼克酰胺腺嘌呤二核苷酸(NADH)为底物,测定NADH在340nm处的吸光度改变以判定乳酸脱氢酶(LDH)的活力。结果表明,所提取的LDH具有明显活力。
出处 《哈尔滨医科大学学报》 CAS 1999年第1期71-72,共2页 Journal of Harbin Medical University
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