HPLC法测定痰咳清片中盐酸麻黄碱的含量及含量均匀度

HPLC Determination of Ephedrine clear phlegm cough content and content uniformity

目的:建立痰咳清片中盐酸麻黄碱的含量及含量均匀度的测定方法。方法:色谱柱为phenomenex ODS C18柱,流动相为乙腈-0.1%磷酸(9∶87),流速为1.0mL·min-1,检测波长为210nm。结果:盐酸麻黄碱在0.256-3.072μg范围内线性关系良好(r=0.9999),平均加样回收率为98.7%(RSD%=1.5%)。结论:该方法简便、准确,可用于该制剂的质量控制。

Objective： To establish a determination of content and content uniformity of the ephedrine hydrochloride in the Slice sputum cough.Methods： The column was phenomenex ODS C18 column, mobile phase of acetonitrile-0.1% phosphoric acid （nine eighty-seven）, flow rate 1.0 mL·min^-1, the detection wavelength was 210 nm.Results： ephedrine hydrochloride in the range of 0.256-3.072 μg good linear relationship （r=0.9999）, the average recovery was 98.7% （RSD%=1.5%）.Conclusion： The method is simple, accurate, and can be used for the preparation of quality control.