Treg/Th17平衡的改变在大鼠原位气管移植急性排斥发生中的作用

The role of unbalance of Treg and Th17 cells in acute rejection after orthotopic tracheal transplantation in rats

目的研究移植排斥急性期Treg与Th17细胞平衡的改变在介导移植排斥中的作用。方法以Lewis大鼠为受体,接受BN大鼠(异系移植组)、Lewis大鼠(同系移植组)气管进行原位移植,未移植的正常Lewis大鼠作为对照。在移植后第7天处死大鼠,取脾脏和颈部淋巴结制备单个核细胞,进行细胞内Foxp3(Treg)或IL-17(Th17)染色后流式细胞仪检测分析。结果移植后第7天异系移植组大鼠与其余两组比较,症状较重,发生较严重免疫排斥反应。流式细胞仪检测结果显示异系移植组脾脏和淋巴结Foxp3^+ Treg比例与其余两组比较未有明显变化;异系移植组脾脏和淋巴结Th17细胞的比例与其余两组比较有显著升高,而同系移植组与正常对照组脾脏和淋巴结Th17细胞比例没有差别。结论急性移植排斥时Treg与Th17细胞比例失衡,Th17细胞分化增强,比例增加,提示Treg/Th17失衡可能是急性期移植排斥发生的重要因素。

Objective To study the role of Treg/Th17 balance in the development of transplantation rejection. Methods Orthotopic tracheal transplantations were performed in a syngeneic （Lewis-to- Lewis） and allogeneic（BN-to-Lewis） setting. Untreated normal Lewis rats were also chosen as control. After 7 days, spleen and lymph nodes were removed and mononuclear cells were prepared, followed by flow cytometry analysis of Foxp3 （Treg） and intracellular IL-17 （Thl7） expression. Results No significant difference was found in Foxp3（Treg） expression between three groups. While, the expression of Th17 cells in allogeneic group were increased compared to syngeneic and nomal control groups. However, there was no significant difference of Th17 cells between syngeneic groups and nomal control group. Conclusion The rate of Th17 is incresed during transplant rejection, and the unbalance between Treg and Th17 cells are related to the acute rejection after orthotopic tracheal transplantation.