跌创散促进兔实验性骨折愈合的作用

Effect of Diechuang San on the experimental fracture healing in rabbits

背景:跌创散有活血祛瘀,消肿止痛之功,对软组织损伤疗效显著。骨折与软组织损伤同属中医"筋伤"的范畴。目的:观察跌创散对实验性骨折愈合作用的影响。方法:取兔龄为6个月左右的雄性新西兰大耳白兔36只,造成双侧桡骨中段约3mm骨缺损模型,造模成功后随机分为模型组和跌创散组。模型组仅给予绷带包扎,跌创散组给予跌创散外敷,每2d更换1次包扎物,分别于造模后2,4,6周观察新西兰大耳白兔桡骨骨折愈合过程中血清碱性磷酸酶、钙、磷浓度变化,骨痂病理组织学改变及不同时期X射线的变化。结果与结论:跌创散组骨缺损填充快,骨折愈合早,前4周血清碱性磷酸酶、钙、磷浓度较高(P<0.05,P<0.01),可以促进钙盐在骨折断端的沉积,对骨折愈合有明显的促进作用,血管较模型组数量多且内径大,骨化中心出现早。因此,推测跌创散可能通过影响血清碱性磷酸酶、钙、磷的浓度变化及促进骨折区域血液循环改善等方面而促进骨痂生长,进而达到促进骨折愈合的目的。

BACKGROUND：The Chinese medicine Diechuang San（DCS） has the property of activating blood circulation and removing stasis,as well as deswelling and alleviating pain,which obtain good outcomes in treating soft tissue injury.Bone fracture and soft tissue injury belong to ＂tendon trauma＂ in traditional Chinese medicine.OBJECTIVE：To study the effect of DCS on experimental fracture healing.METHODS：A total of 36 male New Zealand white rabbits were prepared for 3-mm fracture bone defects models at the middle of both radiuses.Then those rabbits were separated into model control group and DCS treatment group,which were treated by bandaging and external application of DCS,once two days.Afterwards,the changes of alkaline phosphatase（ALP）,Ca and P concentrations,histopathology,as well as the X-ray films were observed at 2,4 and 6 weeks after model preparation.RESULTS AND CONCLUSION：Compared with the model group,the bone defects in the DCS group were filled quickly and the fracture healed earlier.The concentrations of serum ALP,Ca and P were greater in the first 4 weeks（P 0.05,P 0.01）,which could promote the deposition of calcium in the fracture fragments,and promoted the fracture healing.The stimulated blood vessel was greater than the model group with larger inside diameter,and the ossification centers appeared earlier.Therefore,we presume that DCS may influence the serum ALP,Ca,P concentration changes and promote blood circulation to improve the fracture region such as the promotion of callus growth,and thus achieve the purpose of promoting fracture healing.