基因及转基因技术在慢性创面修复中的应用

Application of genetic and transgenic technology in repairing chronic wounds

背景:目前难愈创面是外科救治中的难点,传统的治疗方法效果不佳。有文献表明生长因子在创面愈合中起着重要作用,而转基因疗法是近几年兴起的一种新的治疗方式。目的:通过对近期基因治疗进展的文献综合分析,对近年难愈创面基因治疗的研究现状与进展作一综述。方法:由第一作者检索Pubmed数据库,检索时间范围为1999-01/2008-12,检索英文关键词为"gene therapy,Burns;Chronic wounds"。纳入文章所述内容应与难愈创面、创面基因治疗有关,共保留33篇英文文献进行分析。结果与结论:转基因技术根据转染细胞的来源可以分为体内基因转染和体外基因转染两大类,体内转基因方法具有其难以比拟的优势。转基因方法虽然目前无法在临床中推广,但其拥有广阔的前景。

BACKGROUND：Refractory wound is difficult to treat using traditional methods in the clinic.Studies have shown that growth factor plays an important role in wound healing,and transgenic therapy is emerging to treat refractory wound.OBJECTIVE：To review the research status and progress of treating refractory wound by summarizing literatures concerning gene therapy.METHODS：PubMed database was searched by the first author using key words of ＂gene therapy,burns,chronic wounds＂ for English papers published between January 1999 and December 2008.Documents with regard to refractory wound and gene therapy were included.Totally 33 literatures were analyzed. RESULTS AND CONCLUSION：According to sources of transfected cells,the transgenic technology can be divided into in vivo gene transfection and in vitro gene transfection.The in vivo gene transfection processes greater superiority than other methods.Though it can not be spread in clinical application,transgenic technology owns extensive prospect.