丙交酯-三亚甲基碳酸酯共聚物的生物相容性研究

Biocompatibility Evaluation of Lactide-Trimethylene Carbonate Copolymers

生物相容性是生物材料区别于其它材料的实质所在,它既是生物材料评价的核心,也是设计和改进生物材料的基础。为了评价丙交酯-三亚甲基碳酸酯共聚物的生物相容性,进行了以聚左旋丙交酯(PLLA)和聚三亚甲基碳酸酯(PTMC)为对照的两种共聚物材料聚左旋丙交酯-b-三亚甲基碳酸酯(PLLA-b-PTMC)、聚内消旋丙交酯-b-三亚甲基碳酸酯(PDLLA-b-PTMC)的浸提液试验、材料与细胞的直接接触试验、溶血试验和血小板黏附试验。结果表明,试验的各种材料的细胞毒性均合格,细胞在共聚物材料表面的增殖不如PLLA但优于PTMC;共聚改性后的材料没有引起显著的溶血,其表面血小板黏附数量较PLLA明显降低,血小板的激活程度也有所降低。因此,共聚物PLLA-b-PTMC、PDLLA-b-PTMC的生物相容性与PLLA接近,共聚改性尚未引起聚乳酸的生物相容性发生明显变化,还可能对其有改善作用。

Biocompatibility is the essential property of biomaterials,which is the essence of biomaterial evaluation as well as the foundation of the design and improvement of biomaterials. Several methods were carried out to evaluate the biocompatibility of poly（L-Lactide）-b-poly（trimethylene carbonate （PLLA-b-PTMC） and poly（D,L-Lactide）-b-poly（trimethylene carbonate） （PDLLA-b-PTMC） with poly（L-Lactide） （PLLA） and poly（trimethylene carbonate）（PTMC） as control,including extract liquid experiment,directly contact experiment of materials and cells,hemolytic ratio analysis and platelet adhesion investigation. The results revealed that all the materials exhibited an acceptable cytotoxicity,and proliferation of cells on the modified materials was less than that on the PLLA but more than that on PTMC. The results of hemocompatibility experiments showed that no significant hemolysis was detected when all the materials were in use; in addition,the numbers of platelets adhered on the surface of copolymers were smaller than that on the surface of PLLA,and the degree of platelet deformation was slighter. So,the biocompatibility of copolymers is similar to that of PLLA,the biocompatibility of PLLA is not remarkably changed by modification with PTMC,but rather is improved.