背部微循环观察室活体观测技术及应用

Intravital Observation Technology of Dorsal Microcirculatory Chamber and Its Application

微循环的观测对基础研究和临床诊断具有重要意义。麻醉状态下观测微循环会产生应激反应,影响机体正常生理状态,干扰实验结果。目前,国外采用背部微循环观察室进行清醒状态下的活体观测,可排除麻醉的影响。以国外对此方法的研究应用为基础,结合国内微循环观测的现状,围绕背部微循环观察室的构型和功能、观测技术和应用等方面进行了综述。

Observation of microcirculation plays an important role on the basic research and clinical diagnosis. However,an observation as such on the anesthetized patient will cause stress reaction,thus it will affect normal physiological state and interfere experimental results. At present,a method adopting dorsal microcirculatory chamber （DMC） to do in vivo observation in an unanesthetized state can eliminate the influence of anesthesia. Based on the research reports and practical applications of this method abroad,we summarize,in here,the configuration,function,observation techniques; the application of DMC; and the research states of microcirculation observation.