原子力显微镜与蛋白质研究

Atomic Force Microscopy Involved in Protein Study

原子力显微镜是一种新型纳米显微技术,具有高分辨率(亚纳米级)、无需对样品进行特殊处理、近生理环境下实时观测样品、原位液态环境下观测样品、可在分子水平上研究样品的物理化学特性等优点。近年来,原子力显微镜在蛋白质研究中取得了令人瞩目的进展。本文介绍了原子力显微镜的原理和操作模式,阐述了它在蛋白质成像、吸附、折叠与展开、组装、单分子识别以及其他与蛋白质有关研究中的应用,并展望了它在蛋白质研究中的前景。

Atomic force microscopy is a rather new type of nano microscopic technology. It has some advantages,such as high resolution （sub-nano scale）; avoidance of special sample preparation; real-time detection of samples under nearly physiological environment; in situ study of samples under water environment; feasibility of investigating physical and chemical properties of samples at molecular level,etc. In recent years,the application of atomic force microscopy in protein study has brought about outstanding achievements. In this paper are introduced the principle and operation modes of atomic force microscopy,also presented are its application in protein imaging,adsorption,folding-and-unfolding,assembly,and single molecular recognition. Additionally,the future application of atomic force microscopy in protein study is prospected.