核心蛋白多糖及其mRNA在硬皮病模型小鼠皮损中的表达

Expression of decorin and its mRNA in skin lesion of scleroderma mice

目的观察核心蛋白多糖及其mRNA在正常小鼠皮肤和硬皮病小鼠皮损中的表达，探讨其在硬皮病发病中的作用。方法构建博来霉素诱导的硬皮病小鼠模型，从皮肤和肺组织病理及胶原含量2个方面来验证模型；用免疫组织化学法和反转录-聚合酶链反应（RT—PCR）法测定核心蛋白多糖及其mRNA在硬皮病小鼠及正常小鼠皮肤中的表达。数据采用秩和检验和t检验进行分析。结果根据模型组小鼠和对照组小鼠的皮肤和肺组织病理、皮肤胶原含量的比较，确定硬皮病小鼠模型构建成功。免疫组织化学示：核心蛋白多糖mRNA在对照组没有显著的阳性表达变化；RT—PCR示：核心蛋白多糖在空白对照组小鼠皮肤中呈阳性高表达（0．60±0．15），而在模型组小鼠呈现表达下调[（0．26±0．03），P〈0．05]。结论核心蛋白多糖及其mRNA在硬皮病小鼠中呈低表达；推测这种低表达可能与转化生长因子（TGF）-β1与TGF-β2在硬皮病小鼠中高表达，中和或消耗核心蛋白多糖有关。

Objective To observe the expression of decorin and its mRNA in the skin of normal mice and lesion of scleroderma mice to explore the possible role of decorin in the pathogenesis of scleroderma. Methods Scleroderma mice model induced by Bleomyein were developed and the expression of decorin in the scleroderma mice and normal control mice were determined by immunohistochemistry and RT-PCR. Comparisons between groups were peffonued with rank test and t test. Results According to the comparison of the bistopathology of skin and lung and the skin collagen content between the model group and the control group, the construction of scleroderma model was successful. The results showed that the expression of decorin had no significant change in each group examined by immunohistochemistry, and the expression of decorin had high expression in normal control mice （0.60±0.15） and low expression in scleroderma mice （0.26±0.03） by RT-PCR. Conclusion The low expression of decorin is detected in scleroderma mice. It is suggested that this low expression maybe related to the high expression of TGF-β1 and TGF-β2 in scleroderma mice to neutralize or consume decorin.