复合膳食纤维对实验性溃疡性结肠炎大鼠肠黏膜屏障功能的影响

Effects of dietary fiber complex on intestinal mucosal barrier in rats with experimental ulcerative colitis

目的 观察添加复合膳食纤维（DFC）的肠内营养（EN）对实验性结肠炎大鼠肠黏膜屏障功能的影响.方法 将96只SD大鼠用乙酸灌肠法制成结肠炎模型后随机分成3组,C组给予不含DFC的整蛋白型肠内营养粉剂（商品名：能全素）;T1组和T2组分别给予含整蛋白型肠内营养 粉剂和DFC的EN.DFC由可溶性膳食纤维（SDF）和不溶性膳食纤维（IDF）按一定比例配制,T1组和T2组中,SDF和IDF的比例分别为1：2和1：3.检测EN后第1、3、5、7天大鼠血浆D-乳酸浓度和血浆二胺氧化酶（DAO）活性并取肠道标本观察肠黏膜变化情况.结果 第3、5、7天的T1组与T2 组血浆D-乳酸浓度和DAO活性较C组下降（P〈0.05）,T1组与T2组间差异无统计学意义（P〉0.05）.各时间点T1组与T2组结肠损伤组织学评分均较C组下降（P〈0.05）,第5、7天T2组较T1组评分下降幅度大（P〈0.05）.结论 结肠炎大鼠用含有DFC的EN可降低肠道通透性,对肠黏膜屏障有一定的保护作用,不同比例的SDF和IDF 对肠黏膜保护效果不同,适量增加IDF的比例可以增加这种保护效果.

Objective To evaluate the effect of enteral nutrision （EN） containing dietary fiber complex （DFC） on intestinal mucosal barrier in rats with experimental colitis. Methods Ninety six SD rats were induced into ulcerative colitis by acetic acid enema.than were divided into 3 groups. The rats in group C received non-DFC nutrison, while the rats in group Tl or group T2 received soluble dietary fiber （SDF） and insoluble dietary fiber （IDF） of the EN with different proportion （1：2 or 1：3, respectively）. At the first-, third-,fifth- and seventh-day after the EN, the concentration of D-lactate and diamine oxidase （DAO） activity in plasma were detected and intestinal samples were obtained to observe the changes of intestinal mucosa. Results At the third-,fifth- and seventh-day,the D-lactate concentration and the DAO activity in plasma significantly reduced in group Tl and T2 when compared with group C （P〈0. 05） , but there was no statistical difference between group Tl and group T2. The scores of histological damages of colon in the group Tl and group T2 decreased in comparison with group C at all time points （P 〈 0. 05）. However, the scores of histological damages of colon significantly decreased in group T2 when compared with group Tl at day 5 and day 7 （P 〈0. 05）. Conclusion EN containing DFC may reduce the intestinal permeability of colitis in rats, which has a protective effect on intestinal mucosal barrier. While different proportions of the SDF or IDF ratio result in different effects on the mucosal protection. This protective effect will be enhanced by increasing the ratio of IDF in a certain scale.