选择性抑菌剂对添加稻草红壤旱土团聚体磷素微生物转化的影响

Effects of selective microbial inhibitors on the microbial transformation of phosphorous in aggregates of highly weathered red soil with rice straw amendment

为了进一步探明红壤旱土磷素微生物固持的机理,采用室内模拟培养试验研究了微生物类群对红壤旱土团聚体(0.2～2mm)磷素转化的作用.结果表明:在培养90d期间,添加稻草处理能显著提高红壤旱土团聚体的微生物生物量碳、生物量磷、提取磷(Olsen法)和有机磷的含量.在培养前期(5～30d),与添加稻草处理比较,稻草+真菌抑制剂(放线菌酮)、稻草+细菌抑制剂(四环素+链霉素硫酸盐)处理团聚体微生物生物量碳含量分别降低10.5%～31.8%和6.8%～11.6%,前者的降低幅度显著大于后者(P<0.01),此后加入抑菌剂处理团聚体微生物生物量碳基本保持稳定.添加细菌抑制剂处理团聚体微生物生物量磷含量在培养5～20d期间比加真菌抑制剂处理高10.0%～28.8%,差异显著(P<0.01).表明真菌和细菌均参与红壤旱土团聚体磷素的固持,但真菌的作用明显大于细菌.

In order to further understand the mechanisms of microbial immobilization of phosphorous （P） in highly weathered red soil with organic amendment,an incubation test was conducted to investigate the roles of microbial functional groups in the transformation of P in 0.2-2 mm soil aggregates.Throughout the 90-day incubation period,amendment with rice straw induced a substantial increase in the amounts of microbial biomass C and P,Olsen-P,and organic P in the aggregates.Comparing with rice straw amendment alone,the amendment with rice straw plus fungal inhibitor actidione decreased the amount of microbial biomass C in the aggregates by 10.5%-31.8% in the first 30 days.Such a decrement was significantly larger than that （6.8%-11.6%） in the treatment amended with rice straw plus bacterial inhibitors tetracycline and streptomycin sulphate （P0.01）.After the first 30 days,the microbial biomass C remained constant.In the first 20 days,the amount of microbial biomass P in the aggregates was 10.0%-28.8% higher in the treatment amended with bacterial inhibitors than in the treatment amended with fungal inhibitor （P0.01）.All the results suggested that that both the fungal and the bacterial groups were involved in the microbial immobilization of P in the soil aggregates,and the fungal group played a relatively larger role.