硫对铅污染水稻土微生物活性及群落结构的影响

Effects of sulfur on microbial activity and community structure in a lead-polluted paddy soil

对含有不同浓度铅的水稻土添加硫代硫酸钠进行土培试验,研究硫作用下铅污染水稻土微生物活性及群落结构的变化.结果表明:添加硫底物后,土壤的氧化还原电位(Eh)升高,呼吸强度增强,硫氧化菌的数量显著增加.硫促进了土壤中硫氧化菌等微生物的生长并引起群落结构的变化,克隆测序表明,加硫处理下土壤微生物的特异性条带与拟杆菌、硫杆菌、β-变形菌和嗜酸菌具有很高的相似性.硫处理下土壤碳酸盐结合态及铁锰结合态铅含量发生了显著变化.

A pot experiment was conducted to study the microbial activity and community structure in a lead-polluted paddy soil under effects of amendment with different concentration sodium thiosulfate.The amendment of the sulfur-containing substrate increased the soil oxidation-reduction potential and respiration rate,promoted the growth of soil sulfur-oxidizing bacteria,and induced some changes in soil microbial community structure.The clone sequencing indicated that the specific bands of soil microbes in sodium thiosulfate treatments had very high similarity to Bateroidetes,Thiobacillus,β-proteobacteria,and Acidobacteria.Significant changes were observed in the contents of soil carbonate-and Fe/Mn oxide-bound Pb after the amendment of sodium thiosulfate.