摘要
研究建立经萃取后衍生化高效液相色谱法,用于测定碘化油顺铂乳剂中顺铂含量。方法:乳剂中顺铂由二甲基亚砜(DMSO)萃取后,用二乙基硫代氨基甲酸钠(DDTC)衍生化,其产物可在254nm波长处被检出,以氯化镍为内标物,固定相为UBondapakC18H37化学键合反相固定相,流动相为甲醇∶水(80∶20),流速为1.0ml·min-1。结果:测得平均萃取回收率为78.4%,RSD<5%。结论:应用本法测定乳剂中顺铂含量操作简单,快速。
OBJECTIVE:To establish a derivative HPLC method for quantitative determination of cisplatin in emulsion.METHODS:Cisplatin was extracted by dimethyl sulfoxide(DMSO)from emulsion and reacted with sodium diethyldithiocarbamate(DDTC) producing Pt(DDTC) 2 complex,which was extracted by chloroform and could be detected at 254 nm.Analytical column was U Bondapak C 18 H 37 (5 μm,150×4 mm).The mobile phase was methanol water(80∶20).The flow rate was 1 ml·min -1 ,the internal standard was nickel chloride.RESULTS:The mean recovery of extraction was 78.4% ,the coefficients of variation were not more than 5%.CONCLUSIONS:This method was simple and feasible.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1999年第2期78-80,共3页
Chinese Journal of Hospital Pharmacy