高效液相色谱法测定乳剂中顺铂含量

Determination of cisplatin in emulsion by derivative HPLC

研究建立经萃取后衍生化高效液相色谱法，用于测定碘化油顺铂乳剂中顺铂含量。方法：乳剂中顺铂由二甲基亚砜（ＤＭＳＯ）萃取后，用二乙基硫代氨基甲酸钠（ＤＤＴＣ）衍生化，其产物可在２５４ｎｍ波长处被检出，以氯化镍为内标物，固定相为ＵＢｏｎｄａｐａｋＣ１８Ｈ３７化学键合反相固定相，流动相为甲醇∶水（８０∶２０），流速为１．０ｍｌ·ｍｉｎ－１。结果：测得平均萃取回收率为７８．４％，ＲＳＤ＜５％。结论：应用本法测定乳剂中顺铂含量操作简单，快速。

OBJECTIVE:To establish a derivative HPLC method for quantitative determination of cisplatin in emulsion.METHODS:Cisplatin was extracted by dimethyl sulfoxide(DMSO)from emulsion and reacted with sodium diethyldithiocarbamate(DDTC) producing Pt(DDTC) 2 complex,which was extracted by chloroform and could be detected at 254 nm.Analytical column was U Bondapak C 18 H 37 (5 μm,150×4 mm).The mobile phase was methanol water(80∶20).The flow rate was 1 ml·min -1 ,the internal standard was nickel chloride.RESULTS:The mean recovery of extraction was 78.4% ,the coefficients of variation were not more than 5%.CONCLUSIONS:This method was simple and feasible.