不同激素及其浓度配比对深山蔷薇嫩叶柄再生植株诱导的影响

Effect of Various Hormones with Different Concentration Ratios on the Regeneration Plant from Tender Petioles of Rosa marretii Levl.

以深山蔷薇新生嫩叶柄为外植体,应用均匀设计法筛选其最适合的愈伤组织诱导、愈伤组织芽苗分化和生根的培养基,结果表明,最适合的嫩叶柄愈伤组织诱导的培养基为:LS+6-BA1.80mg·L-1+NAA0.10mg·L-1+2,4-D0.08mg·L-1,诱导率为99.0%;愈伤组织再分化芽苗的培养基为:LS+6-BA3.45mg·L-1+NAA0.05mg·L-1,分化率为99.5%;生根培养基:1/2LS(大量元素)+IAA0.05mg·L-1,生根率达98.0%以上。移栽成活率达96.0%以上。本试验成功建立了深山蔷薇嫩叶柄植株再生体系。

The tender petioles of Rosa marretii Levl. was used as explant. Uniform design was used for selecting the most suitable media for callus induction from tender petioles,callus redifferentiation and regeneration shoots rooting. The results showed that LS＋6-BA 1.80mg·L^-1NAA 0.10mg·L^-12,4-D 0.08mg·L^-1was suitable for callus induction with the induction rate of callus being 99.0%; LS＋6-BA 3.45mg·L^-1NAA 0.05mg·L^-1was suitable for callus redifferentiation with the differentiation rate of callus being 99.5%; 1/2LS（macro-elemenyts）＋IAA 0.05mg·L^-1was suitable for rooting with the rate of rooting being more than 98.0%.Transplant success ratio was more than 96.0%. The experiment results proved that plant regeneration system has been successfully established from the tender petioles of R.marretii Levl..