附加N-乙酰半胱氨酸心停搏液对低温缺血-再灌注大鼠心脏的保护作用

Protective effects of cardioplegia enhanced with N acetylcysteine on the heart in hypothermic ischemia reperfused rats

目的：探讨附加Ｎ－乙酰半胱氨酸（ＮＡＣ）心停搏液对低温缺血再灌注大鼠心脏的保护作用。方法：采用离体左心做功模型，观察附加ＮＡＣ液和Ｓｔ．Ｔｈｏｍａｓ液灌注对大鼠全心低温缺血１８０ｍｉｎ再灌注６０ｍｉｎ的作用，比较两组心脏功能和心肌能量代谢的恢复状况。结果：附加ＮＡＣ组再灌注后心脏功能和高能磷酸腺苷酸代谢的恢复状况明显优于Ｓｔ．Ｔｈｏｍａｓ液灌注组。结论：附加ＮＡＣ可显著改善Ｓｔ．Ｔｈｏｍａｓ液心肌保护作用，ＮＡＣ可能是有价值的心脏停搏液添加剂。

Objective: To investigate the protective effects of cardioplegia enhanced with N acetylcysteine (NAC) on the heart in hypothermic ischemia reperfused rats. Methods: The rat hearts was arrested by multiple dose of NAC enhanced cardioplegia and St. Thomas solution perfusion respectively for 180 min at 20℃ and reperfused for 60 min. The recovery of cardiac function and energy metabolism was observed. Results: The recovery of cardiac function and energy metabolism was much better in the hearts perfused with NAC enhanced cardioplegia than with St. Thomas solution. Conclusion: The enhancement with NAC can significantly improve the myocardial protective effects of St. Thomas solution, which suggests that NAC might be a valuable additive for hypothermic cardioplegic myocardial protection.