发酵农作物秸秆优良菌株木霉L-7发酵条件的优化

Optimization of Fermentation Conditions of the Excellent Strain Trichoderma L-7 for Crops Straw Fermention

研究了木霉L-7利用稻草粉和玉米秸秆粉作为发酵基质进行的固态发酵;探讨了稻草粉和玉米秸秆粉的比例、碳源和氮源的种类、培养基含水量、培养基初始pH值、发酵温度、发酵时间及通气量对其产纤维素酶的影响。结果表明:最佳发酵培养基配方为稻草粉54.0g,玉米秸秆粉27.0g,麸皮19.0g,葡萄糖1.0g,硫酸铵1.0g,磷酸二氢钾0.1g,培养基含水量为1∶3;最优化培养条件为培养基初始pH值6.0,发酵温度30℃,发酵时间6天,通气量为60g/1000mL。在以上条件下,纤维素酶活达到最高。

With rice straw powder and corn straw stem powder as fermentation substrate for solid-phase fermentation,this experiment studies Trichoderma L-7.It discusses the influence of the proportion of rice straw powder and corn straw stem powder,the variety of carbon source and nitrogen source,water content of culture medium,initial pH value of culture medium,fermenting temperature and time,as well as ventilatory capacity on the production of cellulas.The results show that the best fermentation medium contains rice straw powder 54.0g,corn straw stem powder 27.0g,mesh 19.0g,glucose 1.0g,KH2PO4 0.1 g,water content of culture medium 1∶3,The best condition for solid fermentation is initial pH value 6.0,fermentation temperature 30℃,fermentation time 6 days and ventilatory capacity 60 g / 1000 ml.When Trichoderma L-7 is in the best fermentation medium and the best condition,cellulose is the highest.